Chloroquine has been extensively used in mass drug administrations, which may have contributed to the emergence and spread of resistance. It is recommended to check if chloroquine is still effective in the region prior to using it. Chloroquine and prescribing information Order chloroquine Adults will be randomized to receive 1, 3 or 5 bites of Anopheles A. stephensi mosquitoes infected with the NF54 strain of chloroquine-sensitive Plasmodium P. falciparum. Part B will be informed by the results of Part A. Thus, a total of approximately 38 adults will receive a malaria challenge. Aralen chloroquine is an antimalarial drug used for the treatment of malaria and extraintestinal amebiasis. Common side effects are reduced hearing, tinnitus, nausea, vomiting, and diarrhea. Dosage, drug interactions, and pregnancy and breastfeeding safety are provided. P. falciparum strain NF54 PfNF54 was derived from a patient who had never left the Netherlands. PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center MR4 and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine. The Centers for Disease Control and Prevention recommend against treatment of malaria with chloroquine alone due to more effective combinations. In areas where resistance is present, other antimalarials, such as mefloquine or atovaquone, may be used instead. Falciparum nf54 chloroquine Chloroquine Resistance in Plasmodium falciparum - microbewiki, Aralen chloroquine Malaria Drug Side Effects & Dosage Chloroquine solubility Plasmodium falciparum NF54 P. falciparum strain NF54 PfNF54 was derived from a patient who had never left the Netherlands. PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center MR4 and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine. Chloroquine Ligand page IUPHAR/BPS Guide to PHARMACOLOGY. Plasmodium falciparum Malaria Target Species page IUPHAR.. Protection against a Malaria Challenge by Sporozoite.. NF54HT-GFP–luc blood stage parasites grew in in vitro culture comparably to the parent NF54 strain, expressed robust levels of luciferase and were used to calculate the IC 50 values of two known drugs that target blood stage parasites – chloroquine CQ and artesunate AS. Parasites were grown in a 96-well format and treated with increasing concentrations of drug for 72 h 0–400 nm for CQ and 0–100 nm for AS. Since the first documentation of P. falciparum chloroquine resistance in the 1950s, resistant strains have appeared throughout East and West Africa, Southeast Asia, and South America. The effectiveness of chloroquine against P. falciparum has declined as resistant strains of the parasite evolved. They effectively neutralize the drug via a mechanism that drains chloroquine away from the digestive vacuole. Oct 07, 2019 Lange et al. prepared new artemisinin-based hybrid compounds 16 by attaching ferrocene with dihydroartemisinin via piperazine linker, as shown in Figure 4were evaluated against chloroquine-resistant W2 and K1 in vitro, and chloroquine-sensitive NF54 strains of P. falciparum parasites.