This procedure can be modified for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Plaquenil naisea Hydroxychloroquine cardiotoxicity To investigate the effects of chloroquine diphosphate CQ on lung cancer cell growth, we treated A549 cells, a lung cancer cell line, with the drug at various concentrations 0.25–128 μM for 24–72 h. Search results for diphosphate at Sigma-Aldrich. Chloroquine diphosphate salt. 4 μm, ≥80 m 2 g; A severe eye problem has happened with chloroquine. This may lead to lasting eyesight problems. The risk may be higher if you have some types of eye or kidney problems. The risk may also be higher with some doses of chloroquine, if you use chloroquine for longer than 5 years, or if you take certain other drugs like tamoxifen. Store at 4 ℃.*Pro-Tip* Different brands and lots of FBS can promote or inhibit transfection. Last Upload: August 26, 2016Reagents DMEM high glucose L-alanyl-L-glutamine (or alternative stable glutamine)Heat-inactivated FBSLow serum medium such as Opti-MEM or Opti-Pro SFMChloroquine diphosphate, 25 µMPEI, 1 mg/m LMicrocentrifuge tubes10 cm tissue culture dishes Pipettes Pipette tips Hydrochloric acid Sodium hydroxide0.22 μm polyethersulfone (PES) filter0.45 μm PES filter Syringes for filtering Reagent Preparation DMEM Complete: 10% v/v FBS and 4 m M L-alanyl-L-glutamine To a 500 m L bottle of DMEM high glucose, add 55 m L of heat inactivated FBS and 11 m L of 200 m M L-alanyl-L-glutamine. Chloroquine diphosphate 25 μm Chloroquine for research Cell-culture tested InvivoGen, Diphosphate Sigma-Aldrich Can plaquenil effect narcolepsyHydroxychloroquine thyroidPlaquenil teethHydroxychloroquine pediatric dose And 50 mg/kg; i.p.; q.d. for 28 days. Applications. Chloroquine Diphosphate treatment at the doses of 25 and 50 mg/kg significantly reduced the rates of primary tumor growth. In addition, 30% and 60% of mice in the 25 and 50 mg/kg Chloroquine Diphosphate-treated groups still survived on 61st day. Other notes Chloroquine diphosphateAntimalarial drugCAS# 50-63-5. Chloroquine Indications, Side Effects, Warnings -. Antitumor and antimetastatic activities of chloroquine.. How to use Chloroquine Phosphate. Take this medication by mouth, usually with food to prevent stomach upset, exactly as directed by your doctor. Daily or weekly dosing, dosage amount, and length. The cells are cultured in 6-well plates with normal culture medium in the presence of vehicle or 25 or 50 μM chloroquine, until near confluency, after which they are rinsed with sterile phosphate-buffered saline PBS and cultured further for the indicated times in serum-free culture medium. Usual Adult Dose for Malaria Prophylaxis. 500 mg chloroquine phosphate 300 mg base orally on the same day each week Comments-If possible, suppressive therapy should start 2 weeks prior to exposure; if unable to start 2 weeks before exposure, an initial loading dose of 1 g chloroquine phosphate 600 mg base may be taken orally in 2 divided doses, 6 hours apart.