Chloroquine has been extensively used in mass drug administrations, which may have contributed to the emergence and spread of resistance. It is recommended to check if chloroquine is still effective in the region prior to using it. Hydroxychloroquine also known as Venus williams plaquenil Plaquenil and blood sugar Chloroquine is a member of quinolone family and is a weak intercalating agent. Chloroquine is used for treating amebiasis, rheumatoid arthritis, discoid and systemic lupus erythematosus. Application DNA intercalator. Also used to increase transfection efficiency. Chloroquine diphosphate salt has been used • in in vitro antiplasmodial assays Chloroquine was obtained from Sigma C6628, St. Louis, MO. Chloroquine stock solutions were made at 10 mg/ml in double deionized water, and sterilized by filtration with an Acrodisc syringe filter with a 0.2 μm Tuffryn membrane Pall Life Sciences, New York. Aliquots of the sterile stock solutions were stored at -20°C until use. A stock solution is a commercially prepared solution of known concentration and is a commercially prepared solution of known concentration, is often used for this purpose. Diluting a stock solution is preferred because the alternative method, weighing out tiny amounts of solute, is difficult to carry out with a high degree of accuracy. The Centers for Disease Control and Prevention recommend against treatment of malaria with chloroquine alone due to more effective combinations. In areas where resistance is present, other antimalarials, such as mefloquine or atovaquone, may be used instead. Chloroquine stock concentration Chloroquine - Wikipedia, Chloroquine treatment of ARPE-19 cells leads to. - PubMed Central PMC Plaquenil and aps Same final concentration of solvent in test wells used to solubilize the compounds of interest. For positive control Dilute 5 mM Chloroquine stock 10-fold by adding 90 µl EMEM to 10 µl Chloroquine 5 mM, generating 0.5 mM Chloroquine Working Stock. Add 50 µl Working Stock to 104 HepG2 cells in 200 µl media, e.g. EMEM. K977-Glycerol Cell-Based Assay Kit - BioVision. Concentration of Solutions - Chemistry LibreTexts. Chloroquine C18H26ClN3 - PubChem. Sasaki et al 2010 Chloroquine potentiates the anti-cancer effect of 5-fluorouracil on colon cancer cells. BMC Cancer 10 370 PMID 20630104. If you know of a relevant reference for Chloroquine diphosphate, please let us know. Concentration in order to observe changes in autophagic flux. Unused stock Chloroquine may be stored in small aliquots at -20°C for several weeks. 2. 1X Assay Buffer Allow the 10X Assay Buffer to warm to room temperature. Make sure that the reagent is free of any crystallization before dilution. Chloroquine is the generic form of the brand-name prescription medicine Aralen, which is used to prevent and treat malaria — a mosquito-borne disease caused by a parasite — and to treat.