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Tick-borne illnesses – including Lyme disease and rapidly rising cases of anaplasmosis – have become more than a worrisome health threat for people and their pets. Valeria Borovkoff, left, guides Mac, a Rheinland Pfalz Saar horse, and Ali Lindsay guides Gambler, a Canadian Sport Horse, along a path toward the stable at the Cedar Crest Farm Equestrian Center in Pine Plains. In 2012, Rhinebeck Equine veterinary practice prescribed bottles of doxycycline to treat Lyme disease for $36 apiece. Now, each can cost $400 or more — and horses may need six 500-tablet bottles for a 30-day treatment. Horses aren't the only ones affected by a rise in the cost of the prime antibiotic used against Lyme disease. Potentially, so are the 300,000 Americans infected yearly. Pet owners are simply more likely to notice the price hike because they are less likely to have insurance to cover it. 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Therefore, researchers decided to investigate its possible use against osteoarthritis. ACVS, associate professor of veterinary clinical sciences at Cornell University, was the principal investigator on the study.) Previous laboratory research found that at concentrations as low as 0.0462 microliters (ï¿½g/m L) in synovial (joint) fluid and plasma, doxycycline could inhibit the expression of matrix metalloproteinases 3 and 13 (MMP 3 and 13). Various MMP enzymes have been implicated in osteoarthritic and laminitic disease processes, so inhibiting them might help slow these disease processes. However, antibiotics shouldn’t be used at antimicrobial levels (which are substantially higher than 0.0462 microliters (ï¿½g/m L) without good reason, because this might promote antibiotic-resistant bacteria strains. 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